EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.8.5 | expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.8.5 | A383T | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Rattus norvegicus |
1.3.8.5 | E107D/L112V/T115K/I117K | site-directed mutagenesis, mutant is not stable | Rattus norvegicus |
1.3.8.5 | F105L | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Rattus norvegicus |
1.3.8.5 | L220M/A383T | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Rattus norvegicus |
1.3.8.5 | L220M/L222I | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Rattus norvegicus |
1.3.8.5 | L220M/L222I/A383T | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Rattus norvegicus |
1.3.8.5 | L222I/A383T | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Rattus norvegicus |
1.3.8.5 | additional information | construction of deletion mutants, substrate specificity and catalytic efficiency, overview | Rattus norvegicus |
1.3.8.5 | S177N | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Rattus norvegicus |
1.3.8.5 | V104L | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Rattus norvegicus |
1.3.8.5 | V104L/F105L | site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.8.5 | 0.0011 | - |
(S)-2-methylbutanoyl-CoA | mutant L222I/A383T, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.0015 | - |
(S)-2-methylbutanoyl-CoA | deletion mutant M10, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.002 | - |
(S)-2-methylbutanoyl-CoA | mutant L220M/A383T, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.0026 | - |
(S)-2-methylbutanoyl-CoA | mutant F105L, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.0027 | - |
(S)-2-methylbutanoyl-CoA | wild-type enzyme, 32°C | Homo sapiens | |
1.3.8.5 | 0.0039 | - |
(S)-2-methylbutanoyl-CoA | wild-type enzyme, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.0053 | - |
hexanoyl-CoA | mutant L220M/A383T, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.0055 | - |
hexanoyl-CoA | mutant L222I/A383T, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.0107 | - |
(S)-2-methylbutanoyl-CoA | mutant V104L/F105L, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.026 | - |
isobutyryl-CoA | mutant L222I/A383T, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.035 | - |
hexanoyl-CoA | mutant F105L, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.036 | - |
hexanoyl-CoA | wild-type enzyme, 32°C | Homo sapiens | |
1.3.8.5 | 0.044 | - |
hexanoyl-CoA | wild-type enzyme, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.049 | - |
isobutyryl-CoA | mutant L220M/A383T, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.057 | - |
hexanoyl-CoA | deletion mutant M10, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.06 | - |
isobutyryl-CoA | deletion mutant M10, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.085 | - |
isobutyryl-CoA | mutant V104L/F105L, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.095 | - |
isobutyryl-CoA | mutant F105L, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.11 | - |
isobutyryl-CoA | wild-type enzyme, 32°C | Rattus norvegicus | |
1.3.8.5 | 0.13 | - |
isobutyryl-CoA | wild-type enzyme, 32°C | Homo sapiens | |
1.3.8.5 | 0.15 | - |
hexanoyl-CoA | mutant V104L/F105L, 32°C | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.8.5 | Homo sapiens | - |
- |
- |
1.3.8.5 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.8.5 | recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) | Rattus norvegicus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.3.8.5 | 2-methylbutanoyl-CoA + electron-transfer flavoprotein = (E)-2-methylbut-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+ | reaction mechanism determining substrate specificity | Homo sapiens | |
1.3.8.5 | 2-methylbutanoyl-CoA + electron-transfer flavoprotein = (E)-2-methylbut-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+ | reaction mechanism determining substrate specificity | Rattus norvegicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.8.5 | additional information | - |
activity of wild-type and mutant enzymes with different substrates, overview | Rattus norvegicus |
1.3.8.5 | 28 | - |
recombinant wild-type enzyme, substrate (S)-2-methylbutanoyl-CoA | Homo sapiens |
1.3.8.5 | 35 | - |
recombinant wild-type enzyme, substrate (S)-2-methylbutanoyl-CoA | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.5 | (S)-2-methylbutanoyl-CoA + electron-transfer flavoprotein | - |
Homo sapiens | 2-methylbut-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+ | - |
? | |
1.3.8.5 | (S)-2-methylbutanoyl-CoA + electron-transfer flavoprotein | - |
Rattus norvegicus | 2-methylbut-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+ | - |
? | |
1.3.8.5 | hexanoyl-CoA + electron-transfer flavoprotein | 41% of activity with (S)-2-methylbutanoyl-CoA | Homo sapiens | hex-2-enoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
1.3.8.5 | hexanoyl-CoA + electron-transfer flavoprotein | 8% of activity with (S)-2-methylbutanoyl-CoA, wild-type enzyme | Rattus norvegicus | hex-2-enoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
1.3.8.5 | isobutyryl-CoA + electron-transfer flavoprotein | 37% of activity with (S)-2-methylbutanoyl-CoA, wild-type enzyme | Rattus norvegicus | 2-methylacryloyl-CoA + reduced electron-transfer flavoprotein + H+ | - |
? | |
1.3.8.5 | isobutyryl-CoA + electron-transfer flavoprotein | 6% of activity with (S)-2-methylbutanoyl-CoA | Homo sapiens | 2-methylacryloyl-CoA + reduced electron-transfer flavoprotein + H+ | - |
? | |
1.3.8.5 | additional information | substrate specificity of wild-type and mutants, overview | Rattus norvegicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.8.5 | More | structure-function relationship, molecular modeling | Homo sapiens |
1.3.8.5 | More | structure-function relationship, molecular modeling | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.8.5 | SBCAD | - |
Homo sapiens |
1.3.8.5 | SBCAD | - |
Rattus norvegicus |
1.3.8.5 | short/branched chain acyl-CoA dehydrogenase | - |
Homo sapiens |
1.3.8.5 | short/branched chain acyl-CoA dehydrogenase | - |
Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.8.5 | 32 | - |
assay at | Homo sapiens |
1.3.8.5 | 32 | - |
assay at | Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.8.5 | 1200 | - |
hexanoyl-CoA | mutant L222I/A383T, 32°C | Rattus norvegicus | |
1.3.8.5 | 1200 | - |
(S)-2-methylbutanoyl-CoA | mutant L222I/A383T, 32°C | Rattus norvegicus | |
1.3.8.5 | 1800 | - |
hexanoyl-CoA | wild-type enzyme, 32°C | Rattus norvegicus | |
1.3.8.5 | 2000 | - |
isobutyryl-CoA | deletion mutant M10, 32°C | Rattus norvegicus | |
1.3.8.5 | 2000 | - |
hexanoyl-CoA | mutant V104L/F105L, 32°C | Rattus norvegicus | |
1.3.8.5 | 2100 | - |
isobutyryl-CoA | mutant V104L/F105L, 32°C | Rattus norvegicus | |
1.3.8.5 | 2100 | - |
(S)-2-methylbutanoyl-CoA | mutant V104L/F105L, 32°C | Rattus norvegicus | |
1.3.8.5 | 2500 | - |
hexanoyl-CoA | mutant F105L, 32°C | Rattus norvegicus | |
1.3.8.5 | 2600 | - |
(S)-2-methylbutanoyl-CoA | mutant F105L, 32°C | Rattus norvegicus | |
1.3.8.5 | 2700 | - |
isobutyryl-CoA | mutant L220M/A383T, 32°C | Rattus norvegicus | |
1.3.8.5 | 2900 | - |
isobutyryl-CoA | mutant F105L, 32°C | Rattus norvegicus | |
1.3.8.5 | 2900 | - |
isobutyryl-CoA | wild-type enzyme, 32°C | Homo sapiens | |
1.3.8.5 | 3000 | - |
(S)-2-methylbutanoyl-CoA | wild-type enzyme, 32°C | Rattus norvegicus | |
1.3.8.5 | 4000 | - |
(S)-2-methylbutanoyl-CoA | mutant L220M/A383T, 32°C | Rattus norvegicus | |
1.3.8.5 | 5100 | - |
(S)-2-methylbutanoyl-CoA | deletion mutant M10, 32°C | Rattus norvegicus | |
1.3.8.5 | 5800 | - |
hexanoyl-CoA | mutant L220M/A383T, 32°C | Rattus norvegicus | |
1.3.8.5 | 7400 | - |
hexanoyl-CoA | deletion mutant M10, 32°C | Rattus norvegicus | |
1.3.8.5 | 7600 | - |
hexanoyl-CoA | wild-type enzyme, 32°C | Homo sapiens | |
1.3.8.5 | 8100 | - |
isobutyryl-CoA | mutant L222I/A383T, 32°C | Rattus norvegicus | |
1.3.8.5 | 8500 | - |
isobutyryl-CoA | wild-type enzyme, 32°C | Rattus norvegicus | |
1.3.8.5 | 9700 | - |
(S)-2-methylbutanoyl-CoA | wild-type enzyme, 32°C | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.8.5 | FAD | - |
Rattus norvegicus |